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Image Search Results
Journal: Obesity Facts
Article Title: The Role of MKP-5 in Adipocyte-Macrophage Interactions during Obesity
doi: 10.1159/000505343
Figure Lengend Snippet: MKP-5 overexpression in macrophages is involved in M1-to-M2 macrophage polarization. A 500 μM palmitate (PA) was used to treat Raw264.7 cells, and then IL-6, IL-1β, IL-12, and MKP-5 expression was detected at 1, 3, and 6 h by quantitative real-time (qRT)-PCR. B A stably transfected MKP-5 Raw264.7 cell line was constructed, and MKP-5 overexpression was assessed by qRT-PCR and Western blotting. C Raw-PC and Raw-MKP5 cells were stimulated with the indicated concentration of IFN-γ and LPS for 6 h, and M1 markers were measured by qRT-PCR. D Raw-PC and Raw-MKP5 cells were stimulated using IL-4 for 12 h, and M2 markers were measured by qRT-PCR. E, F With 500 μM PA for 6 h, the expression levels of M1 (E) and M2 markers (F) in Raw-PC and Raw-MKP5 cells were detected by qRT-PCR. Results are means ± SEM of triplicate experiments. EtOH, ethyl alcohol. * p < 0.05.
Article Snippet:
Techniques: Over Expression, Expressing, Quantitative RT-PCR, Stable Transfection, Transfection, Construct, Western Blot, Concentration Assay